About MPS

PacBio HiFiLong, accurate reads Oxford NanoporeONT ultra-long reads Hi-CChromatin contacts v3jf13 pseudochromosomes

Raw sequencing data — PacBio HiFi long reads, Oxford Nanopore (ONT) ultra-long reads and Hi-C chromatin-contact reads. The v3jf assembly was built from these three read sets.

The organism: a mulberry psyllid

Paurocephala sauteri is a jumping plant louse (Hemiptera: Sternorrhyncha: Psylloidea) that feeds on the phloem of mulberry (Morus). Nymphs and adults gather on young leaves and shoots; heavy infestations curl and yellow the foliage and coat it with honeydew, which lowers the quality of the leaves harvested for silkworm rearing.

The assembly: chromosome-level

The 1.03 Gb genome was assembled from PacBio HiFi and Oxford Nanopore (ONT) long reads together with Hi-C chromatin-contact data into 13 pseudochromosomes spanning 886.0 Mb (85.7% of the assembly); the remaining 147.3 Mb is placed on 2,448 unanchored sequences. BUSCO completeness is 92.2% against the Hemiptera set (n = 2,510).

The annotation: integrated evidence

20,591 protein-coding genes with 22,977 transcripts and 126,222 exons. Functional evidence covers 12,970 genes by GO, 14,904 by InterPro and 11,430 by eggNOG; 3,164 tRNAs, 606 rRNAs, 240 other non-coding RNAs and 471 pseudogenes complete the annotation.

Length of the 13 pseudochromosomesBar chart of chromosome length in megabases: Chr01 98.2 Mb, Chr02 90.5 Mb, Chr03 86.1 Mb, Chr04 82.7 Mb, Chr05 81.8 Mb, Chr06 76.5 Mb, Chr07 66.5 Mb, Chr08 61.2 Mb, Chr09 55.0 Mb, Chr10 53.2 Mb, Chr11 51.2 Mb, Chr12 49.8 Mb, Chr13 33.4 Mb.025507510098.2Chr0190.5Chr0286.1Chr0382.7Chr0481.8Chr0576.5Chr0666.5Chr0761.2Chr0855.0Chr0953.2Chr1051.2Chr1149.8Chr1233.4Chr13Length (Mb)Total 886.0 Mb
13 pseudochromosomes — length of each chromosome in Mb, summing to 886.0 Mb. Chr01 is the largest (98.2 Mb) and Chr13 the smallest (33.4 Mb).
Functional annotation coverage of the protein-coding genesHorizontal bars out of 20,591 protein-coding genes: GO 12,970 genes (63.0%); InterPro 14,904 genes (72.4%); eggNOG 11,430 genes (55.5%).GO12,970 (63.0%)InterPro14,904 (72.4%)eggNOG11,430 (55.5%)010,29520,591Genes with at least one hit20,591 protein-coding genes
Functional annotation coverage — number of the 20,591 protein-coding genes with at least one GO term (12,970), InterPro signature (14,904) or eggNOG orthologous group (11,430).

Genome Overview

A chromosome-level, HiFi-based assembly of the mulberry psyllid with integrated functional annotation.

Tool Center

Browse, align, search and download the Psauteri_v3jf genome. Each tool opens with a link back to this page.

Gene Search
MPS Annotation API
Look up genes by ID or functional keyword (description / GO / InterPro / KEGG) and inspect their annotation.
Open tool
BLAST
SequenceServer
Run sequence similarity searches against the P. sauteri genome, CDS and protein sets, with nucleotide and protein query types.
Open tool
Genome Browser
JBrowse 2
Interactively browse the 13 pseudochromosomes, the gene models and the annotated repeats of Psauteri_v3jf.
Open tool
Data Downloads
Psauteri_v3jf v1.0 release package
Download the genome FASTA, GFF3 gene models, protein and CDS sequences, the 20-column gene information table and the BLAST databases.
Open tool
API Access
JSON over HTTPS
Query assembly statistics, gene records, chromosomes and the download catalogue programmatically from the read-only JSON API.
Open tool
Planned
Expression Atlas
RNA-seq TPM matrix
Profile expression across developmental stages and tissues once the RNA-seq quantification matrix is released.
In development
Planned
HMMER Search
HMMER 3.4 · Pfam-A
Search homologs (phmmer), annotate domains (hmmscan) or mine gene families (hmmsearch) across the psyllid proteome.
In development
Planned
Synteny & Orthologs
Cross-species comparison
Compare the P. sauteri chromosomes with other Sternorrhyncha genomes and trace conserved gene order.
In development

Research Team & Data Availability

The genome was assembled, annotated and released by the mulberry research group at Southwest University.

Project and contact

Sequencing, assembly, annotation and database: the mulberry research group, State Key Laboratory of Resource Insects, Southwest University, Chongqing, China.

* Correspondence: fanwei2034@163.com

Data availability

  • Genome assembly — FASTA, 1.03 Gb in 2,461 sequences
  • Gene models — GFF3, 20,591 protein-coding genes
  • Protein and CDS FASTA, plus a 20-column gene information table
  • BLAST databases, a JBrowse 2 browser and a JSON API

Psauteri_v3jf v1.0 — assembly release_v3jf_final_v6c, annotation v3jf.merged.20261024. Provided for research use.